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transwell® polyester membrane cell culture inserts  (Corning Life Sciences)

 
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    Structured Review

    Corning Life Sciences transwell® polyester membrane cell culture inserts
    Transwell® Polyester Membrane Cell Culture Inserts, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/transwell+polyester+membrane+cell+culture+insert/96+well+plates/pm40571267-85-24-37
    Average 90 stars, based on 1 article reviews
    transwell® polyester membrane cell culture inserts - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Membrane:

    Article Title: Mice lacking DYRK2 exhibit congenital malformations with lung hypoplasia and altered Foxf1 expression gradient
    Article Snippet: .. The lungs were placed on a Transwell polyester membrane cell culture insert (Corning) and cultured at the air liquid interface in DMEM/ Ham’s F12 medium (Nacalai tesque) supplemented with 10% FBS, penicillin-streptomycin (Nacalai tesque), and Amphotericin B (Sigma) with or without 50 μM Harmine (Tokyo Chemical Industry Co., Ltd.) or 14.8 nM smoothened agonist (SAG) (Enzo Life Sciences). ..

    Article Title: PCL–DOX microdroplets: an evaluation of the enhanced intracellular delivery of doxorubicin in metastatic cancer cells via in silico and in vitro approaches
    Article Snippet: Researchers promoted polymer microspheres in medical engineering and biotechnology such as embedded drug delivery of poly lactic acid microspheres.. Polycaprolactone (PCL) is kind of semicrystalline polymer which was utilized in many varieties of organic solvents.. Therefore, PCL was made as drug-controlled releasing carriers for long-term treatment.

    Article Title: Reactive oxygen species (ROS)-responsive size-reducible nanoassemblies for deeper atherosclerotic plaque penetration and enhanced macrophage-targeted drug delivery
    Article Snippet: .. MAECs (5.0 × 10 4 ) were seeded on a Transwell polyester membrane cell culture insert in 24-well plate (pore size 0.4 μm, diameter 6.5 mm, Corning Incorporated, Corning, NY, USA). .. The transendothelial electrical resistance (TEER) values of the endothelium monolayer were measured by using an electrical resistance meter (Millicell®-Electrical Resistance System, Millipore, USA), and a continuous endothelium monolayer formed until the cell monolayer exhibited a steady TEER value [ , ].

    Article Title: Astrocyte-selective AAV gene therapy through the endogenous GFAP promoter results in robust transduction in the rat spinal cord following injury
    Article Snippet: Sprague–Dawley rats (10–15 days postnatal) were euthanized (two pups per culture) and the spinal cords were carefully removed and embedded in liquid 4% agar and placed on ice to set. .. The block was cut into 400 μm thick sections using a McIlwain Tissue Chopper (Ted Pella, Inc.) and transferred to a six-well plate transwell polyester membrane cell culture insert (Corning, Sigma). ..

    Article Title: SARS-CoV-2 and HIV: Impact on Pulmonary Epithelial Cells
    Article Snippet: .. For the purposes of this study, cells were grown on transwell polyester or polycarbonate membrane cell culture inserts in 6-well plates (24 mm diameter, 0.4 um pore, polyester: Corning Carlsbad, California, USA, cat# 3450; polycarbonate: Corning, cat# 3412) or 100 mm dishes (75 mm diameter, 0.4 um pore, polycarbonate, Corning cat# 7910). ..

    Article Title: Production of reactive species in alginate hydrogels for cold atmospheric plasma-based therapies
    Article Snippet: SaOs-2 were detached from the flask using trypsin (Invitrogen, Thermofisher) and 10000 cells/well were seeded into 24-well plates with 1 mL volume of culture medium. .. After 6 h-adhesion, plasma-treated sterile alginate hydrogels, previously prepared in sterile conditions, were introduced into a CORNING Transwell polyester membrane cell culture insert and placed in suspension in the well, to evaluate the effect of kINPen and APPJ plasma treatment of the alginate hydrogels on the SaOs-2 cell viability. ..

    Article Title: Uterus‐Mimetic Extracellular Microenvironment for Engineering Female Reproductive System
    Article Snippet: Addressing the complexities and challenges inherent in endometrial biology and regeneration, this study develops a uterus-mimetic microenvironment to reconstitute the female reproductive system.. Leveraging a decellularized uterus extracellular matrix (UEM), this approach supports the development and functions of endometrial organoids (EOs) more effectively than the current gold standard, Matrigel.. Extensive proteomic analysis across eight different tissuederived matrices reveals that those closely mirroring the protein profile of UEM–especially those abundant in proteins specific to the female reproductive system–more effectively support EO growth.

    Article Title: Extracellular vesicles of U937 macrophage cell line infected with DENV-2 induce activation in endothelial cells EA.hy926
    Article Snippet: EA.hy926 endothelial cells (ATCC ® CRL-2922 TM ) were maintained under non-pyrogenic conditions using DMEM with sodium pyruvate (Biowest) supplemented with 10% FBS and 1% PS. .. At 80% confluence, cells were trypsinized and 60,000 cells were seeded in the apical domain (upper chamber) of a Corning ® Transwell ® polyester membrane cell culture insert (Sigma-Aldrich) previously treated with Collagen IV (Sigma-Aldrich) and Fibronectin (Invitrogen) and cultured at 37°C and 5% CO 2 during 12 h. At this point and using a voltmeter Millicell ERS-2 (Millipore), initial TEER values were measured (time 0). ..

    Cell Culture:

    Article Title: Mice lacking DYRK2 exhibit congenital malformations with lung hypoplasia and altered Foxf1 expression gradient
    Article Snippet: .. The lungs were placed on a Transwell polyester membrane cell culture insert (Corning) and cultured at the air liquid interface in DMEM/ Ham’s F12 medium (Nacalai tesque) supplemented with 10% FBS, penicillin-streptomycin (Nacalai tesque), and Amphotericin B (Sigma) with or without 50 μM Harmine (Tokyo Chemical Industry Co., Ltd.) or 14.8 nM smoothened agonist (SAG) (Enzo Life Sciences). ..

    Article Title: PCL–DOX microdroplets: an evaluation of the enhanced intracellular delivery of doxorubicin in metastatic cancer cells via in silico and in vitro approaches
    Article Snippet: Researchers promoted polymer microspheres in medical engineering and biotechnology such as embedded drug delivery of poly lactic acid microspheres.. Polycaprolactone (PCL) is kind of semicrystalline polymer which was utilized in many varieties of organic solvents.. Therefore, PCL was made as drug-controlled releasing carriers for long-term treatment.

    Article Title: Reactive oxygen species (ROS)-responsive size-reducible nanoassemblies for deeper atherosclerotic plaque penetration and enhanced macrophage-targeted drug delivery
    Article Snippet: .. MAECs (5.0 × 10 4 ) were seeded on a Transwell polyester membrane cell culture insert in 24-well plate (pore size 0.4 μm, diameter 6.5 mm, Corning Incorporated, Corning, NY, USA). .. The transendothelial electrical resistance (TEER) values of the endothelium monolayer were measured by using an electrical resistance meter (Millicell®-Electrical Resistance System, Millipore, USA), and a continuous endothelium monolayer formed until the cell monolayer exhibited a steady TEER value [ , ].

    Article Title: Astrocyte-selective AAV gene therapy through the endogenous GFAP promoter results in robust transduction in the rat spinal cord following injury
    Article Snippet: Sprague–Dawley rats (10–15 days postnatal) were euthanized (two pups per culture) and the spinal cords were carefully removed and embedded in liquid 4% agar and placed on ice to set. .. The block was cut into 400 μm thick sections using a McIlwain Tissue Chopper (Ted Pella, Inc.) and transferred to a six-well plate transwell polyester membrane cell culture insert (Corning, Sigma). ..

    Article Title: SARS-CoV-2 and HIV: Impact on Pulmonary Epithelial Cells
    Article Snippet: .. For the purposes of this study, cells were grown on transwell polyester or polycarbonate membrane cell culture inserts in 6-well plates (24 mm diameter, 0.4 um pore, polyester: Corning Carlsbad, California, USA, cat# 3450; polycarbonate: Corning, cat# 3412) or 100 mm dishes (75 mm diameter, 0.4 um pore, polycarbonate, Corning cat# 7910). ..

    Article Title: Production of reactive species in alginate hydrogels for cold atmospheric plasma-based therapies
    Article Snippet: SaOs-2 were detached from the flask using trypsin (Invitrogen, Thermofisher) and 10000 cells/well were seeded into 24-well plates with 1 mL volume of culture medium. .. After 6 h-adhesion, plasma-treated sterile alginate hydrogels, previously prepared in sterile conditions, were introduced into a CORNING Transwell polyester membrane cell culture insert and placed in suspension in the well, to evaluate the effect of kINPen and APPJ plasma treatment of the alginate hydrogels on the SaOs-2 cell viability. ..

    Article Title: Uterus‐Mimetic Extracellular Microenvironment for Engineering Female Reproductive System
    Article Snippet: Addressing the complexities and challenges inherent in endometrial biology and regeneration, this study develops a uterus-mimetic microenvironment to reconstitute the female reproductive system.. Leveraging a decellularized uterus extracellular matrix (UEM), this approach supports the development and functions of endometrial organoids (EOs) more effectively than the current gold standard, Matrigel.. Extensive proteomic analysis across eight different tissuederived matrices reveals that those closely mirroring the protein profile of UEM–especially those abundant in proteins specific to the female reproductive system–more effectively support EO growth.

    Article Title: Extracellular vesicles of U937 macrophage cell line infected with DENV-2 induce activation in endothelial cells EA.hy926
    Article Snippet: EA.hy926 endothelial cells (ATCC ® CRL-2922 TM ) were maintained under non-pyrogenic conditions using DMEM with sodium pyruvate (Biowest) supplemented with 10% FBS and 1% PS. .. At 80% confluence, cells were trypsinized and 60,000 cells were seeded in the apical domain (upper chamber) of a Corning ® Transwell ® polyester membrane cell culture insert (Sigma-Aldrich) previously treated with Collagen IV (Sigma-Aldrich) and Fibronectin (Invitrogen) and cultured at 37°C and 5% CO 2 during 12 h. At this point and using a voltmeter Millicell ERS-2 (Millipore), initial TEER values were measured (time 0). ..

    Encapsulation:

    Article Title: PCL–DOX microdroplets: an evaluation of the enhanced intracellular delivery of doxorubicin in metastatic cancer cells via in silico and in vitro approaches
    Article Snippet: Researchers promoted polymer microspheres in medical engineering and biotechnology such as embedded drug delivery of poly lactic acid microspheres.. Polycaprolactone (PCL) is kind of semicrystalline polymer which was utilized in many varieties of organic solvents.. Therefore, PCL was made as drug-controlled releasing carriers for long-term treatment.

    Pore Size:

    Article Title: PCL–DOX microdroplets: an evaluation of the enhanced intracellular delivery of doxorubicin in metastatic cancer cells via in silico and in vitro approaches
    Article Snippet: Researchers promoted polymer microspheres in medical engineering and biotechnology such as embedded drug delivery of poly lactic acid microspheres.. Polycaprolactone (PCL) is kind of semicrystalline polymer which was utilized in many varieties of organic solvents.. Therefore, PCL was made as drug-controlled releasing carriers for long-term treatment.

    Article Title: Reactive oxygen species (ROS)-responsive size-reducible nanoassemblies for deeper atherosclerotic plaque penetration and enhanced macrophage-targeted drug delivery
    Article Snippet: .. MAECs (5.0 × 10 4 ) were seeded on a Transwell polyester membrane cell culture insert in 24-well plate (pore size 0.4 μm, diameter 6.5 mm, Corning Incorporated, Corning, NY, USA). .. The transendothelial electrical resistance (TEER) values of the endothelium monolayer were measured by using an electrical resistance meter (Millicell®-Electrical Resistance System, Millipore, USA), and a continuous endothelium monolayer formed until the cell monolayer exhibited a steady TEER value [ , ].

    Article Title: Uterus‐Mimetic Extracellular Microenvironment for Engineering Female Reproductive System
    Article Snippet: Addressing the complexities and challenges inherent in endometrial biology and regeneration, this study develops a uterus-mimetic microenvironment to reconstitute the female reproductive system.. Leveraging a decellularized uterus extracellular matrix (UEM), this approach supports the development and functions of endometrial organoids (EOs) more effectively than the current gold standard, Matrigel.. Extensive proteomic analysis across eight different tissuederived matrices reveals that those closely mirroring the protein profile of UEM–especially those abundant in proteins specific to the female reproductive system–more effectively support EO growth.

    Blocking Assay:

    Article Title: Astrocyte-selective AAV gene therapy through the endogenous GFAP promoter results in robust transduction in the rat spinal cord following injury
    Article Snippet: Sprague–Dawley rats (10–15 days postnatal) were euthanized (two pups per culture) and the spinal cords were carefully removed and embedded in liquid 4% agar and placed on ice to set. .. The block was cut into 400 μm thick sections using a McIlwain Tissue Chopper (Ted Pella, Inc.) and transferred to a six-well plate transwell polyester membrane cell culture insert (Corning, Sigma). ..

    Clinical Proteomics:

    Article Title: Production of reactive species in alginate hydrogels for cold atmospheric plasma-based therapies
    Article Snippet: SaOs-2 were detached from the flask using trypsin (Invitrogen, Thermofisher) and 10000 cells/well were seeded into 24-well plates with 1 mL volume of culture medium. .. After 6 h-adhesion, plasma-treated sterile alginate hydrogels, previously prepared in sterile conditions, were introduced into a CORNING Transwell polyester membrane cell culture insert and placed in suspension in the well, to evaluate the effect of kINPen and APPJ plasma treatment of the alginate hydrogels on the SaOs-2 cell viability. ..

    Sterility:

    Article Title: Production of reactive species in alginate hydrogels for cold atmospheric plasma-based therapies
    Article Snippet: SaOs-2 were detached from the flask using trypsin (Invitrogen, Thermofisher) and 10000 cells/well were seeded into 24-well plates with 1 mL volume of culture medium. .. After 6 h-adhesion, plasma-treated sterile alginate hydrogels, previously prepared in sterile conditions, were introduced into a CORNING Transwell polyester membrane cell culture insert and placed in suspension in the well, to evaluate the effect of kINPen and APPJ plasma treatment of the alginate hydrogels on the SaOs-2 cell viability. ..

    Suspension:

    Article Title: Production of reactive species in alginate hydrogels for cold atmospheric plasma-based therapies
    Article Snippet: SaOs-2 were detached from the flask using trypsin (Invitrogen, Thermofisher) and 10000 cells/well were seeded into 24-well plates with 1 mL volume of culture medium. .. After 6 h-adhesion, plasma-treated sterile alginate hydrogels, previously prepared in sterile conditions, were introduced into a CORNING Transwell polyester membrane cell culture insert and placed in suspension in the well, to evaluate the effect of kINPen and APPJ plasma treatment of the alginate hydrogels on the SaOs-2 cell viability. ..



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